TY - JOUR AU - Mukhopadhyay, Debanjan AU - Dasgupta, Pinakpani AU - Sinha Roy, Debalina AU - Palchoudhuri, Shauroseni AU - Chatterjee, Ipsita AU - Ali, Shahnaz AU - Ghosh Dastidar, Sujata PY - 2015/08/04 Y2 - 2024/03/29 TI - A Sensitive In vitro Spectrophotometric Hydrogen Peroxide Scavenging Assay using 1,10-Phenanthroline JF - Free Radicals and Antioxidants JA - Free Rad. Antiox. VL - 6 IS - 1 SE - Original Article DO - 10.5530/fra.2016.1.15 UR - https://www.antiox.org/index.php/fra/article/view/74 SP - 124-132 AB - <p><strong>Introduction:</strong> Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is a biologically important, non-radical reactive oxygen species (ROS) that can influence several cellular processes. Ability of anti-oxidants to scavenge H<sub>2</sub>O<sub>2</sub> can be measured by several methods but all the methods are suffering from several lacunae due to poor reproducibility, lack of specificity, inaccuracy, high cost etc. <strong>Objective:</strong> The present study aimed towards development of a rapid, low cost, reproducible, specific as well as sensitive and accurate method to detect H<sub>2</sub>O<sub>2</sub> scavenging activity of anti-oxidants. <strong>Methods:</strong> We have used 1,10-phenanthroline and ferrous ammonium sulphate to detect H<sub>2</sub>O<sub>2</sub> scavenging activity of anti-oxidants.<strong> Results:</strong> We revealed that our assay is able to detect the <em>in vitro</em> H<sub>2</sub>O<sub>2</sub> scavenging activity of both phenolic and non-phenolic anti-oxidants. Furthermore, we found that this assay is highly specific, as scavengers of other types of ROS were unable to show any detectable effect on H<sub>2</sub>O<sub>2</sub> through this assay. Finally, we tested commercially available non-steroidal anti-inflammatory drugs that are known to possess H<sub>2</sub>O<sub>2</sub> scavenging activity and in such drugs also we were able to detect their H<sub>2</sub>O<sub>2</sub> scavenging activity.<strong> Conclusion:</strong> In conclusion, the proposed spectrophotometric method was rapid, cost effective, reproducible and highly specific to detect <em>in vitro</em> H<sub>2</sub>O<sub>2</sub> scavenging activity of diverse compounds.</p> ER -