TY - JOUR AU - M, Govindappa AU - N, Bharath AU - HB, Shruthi AU - Santoyo, Gustavo PY - 2020/12/11 Y2 - 2024/03/28 TI - In vitro Antioxidant Activity and Phytochemical Screening of Endophytic Extracts of Crotalaria pallida JF - Free Radicals and Antioxidants JA - Free Rad. Antiox. VL - 1 IS - 3 SE - Original Article DO - 10.5530/ax.2011.3.11 UR - https://www.antiox.org/index.php/fra/article/view/282 SP - 79-85 AB - <p><strong>Introduction:</strong> The species <em>Crotalaria pallida</em>, which belongs to the Fabaceae family (Sub-family Faboideae), the members of which are herbs, shrubs and trees found in both temperate and tropical areas. All parts of the plants were incubated to know and isolate the endophytes. Antioxidants play an important role in protecting cellular damage by reactive oxygen species. Phenolic compounds from plants or endophytes have been reported to possess strong antioxidant properties. <strong>Results:</strong> Four different endophytic fungi isolated from <em>Crotalaria pallida</em> were tested for various phytochemicals. <em>Aspergillus niger</em> and <em>Fusarium oxysporum</em> yielded the tannin, flavonoids, tepenoids, phenol and saponins from ethanol extract. All four different endophytic extracts were used to evaluated <em>in vitro</em> antioxidant activity by ABTS, DPPH and FRAP method. Antioxidant compounds like total phenol and flavonoid were also determined. The ethanol extracts of <em>A. niger</em> and <em>F. oxysporum</em> showed potent antioxidant activity against ABTS, FRAP and DPPH radicals with EC50. The total amount of phenol and flavonoid quantified were of 19.20, 19.23 gallic acid equivalent per gram of two endophytic fungi, <em>A. niger</em> and <em>F. oxysporum</em> and 7.25 and 6.41 μg/mg of quercetin equivalent respectively. Conclusions: The antioxidant potential may be directly linked to the phenolic compounds present in the endophytes,<em> A. niger</em> and <em>F. oxysporum</em> of <em>Crotalaria pallida.</em> The outcome of the present investigation clearly indicates that <em>A. niger</em> and <em>F. oxysporum</em> showed potential phytochemicals and they can be used as antioxidants.</p> ER -