TY - JOUR AU - Ö, Erol-Dayi AU - M, Pekmez AU - M, Bona AU - A, Aras-Perk AU - N, Arda PY - 2020/12/11 Y2 - 2024/03/28 TI - Total Phenolic Contents, Antioxidant Activities and Cytotoxicity of Three Centaurea Species: C. calcitrapa subsp. calcitrapa, C. ptosimopappa and C. spicata JF - Free Radicals and Antioxidants JA - Free Rad. Antiox. VL - 1 IS - 2 SE - Research Article DO - 10.5530/ax.2011.2.7 UR - https://www.antiox.org/index.php/fra/article/view/176 SP - 31-36 AB - <p><strong>Background:</strong> Many species of the genus<em> Centaurea</em> have been reported to be used in Anatolian folk medicine. In the present study, methanolic and aqueous extracts of three species of <em>Centaurea</em> <em>L</em>. (<em>C. calcitrapa subsp. calcitrapa, C. ptosimopappa and C. spicata</em>) have been assessed for antioxidant and cytotoxic activities. <strong>Materials and Methods:</strong> Antioxidant activity was investigated by DPPH and superoxide anion scavenging and anti-lipid peroxidation assays. Cytotoxic effect on HeLa and Vero cells were investigated by MTT assay. <strong>Results:</strong> Superoxide anion scavenging activities of aqueous extracts were higher than those of methanolic extracts, and the highest activity was detected in the aqueous extracts of<em> C. calcitrapa subsp. calcitrapa</em> with the IC<sub>50</sub> value of 25.91 μg/mL. The methanolic extract of <em>C. ptosimopappa</em> showed the strongest anti-lipid peroxidation activity. Total phenolic contents (mg gallic acid equivalent per gram) were ranged between 17.25 and 120.50 and the highest value was detected in aqueous extract of <em>C. ptosimopappa</em>. The highest cytotoxic activity was observed in the methanolic extract of <em>C. calcitrapa subsp. calcitrapa</em> on both cell lines with the IC<sub>50</sub> values of &lt;100 μg/mL.<strong> Conclusions:</strong> According to the results of activity tests, the selected <em>Centaurea</em> species, especially<em> C. calcitrapa subsp. calcitrapa</em> can be regarded as a potential source of natural antioxidants in the prevention and treatment of diseases in which oxidants or free radicals are implicated.</p> ER -