TY - JOUR AU - Chidoka Chikezie, Paul PY - 2020/12/11 Y2 - 2024/03/28 TI - Levels of Two Oxidative Stress Indicators of Human Sickle Erythrocytes Incubated in Aqueous Extracts of Anacardium occidentale, Psidium guajava and Terminalia catappa JF - Free Radicals and Antioxidants JA - Free Rad. Antiox. VL - 1 IS - 1 SE - Original Article DO - 10.5530/ax.2011.1.7 UR - https://www.antiox.org/index.php/fra/article/view/153 SP - 34-41 AB - <p>An <em>in vitro</em> study was carried out to investigate levels of oxidative stress indicators of sickle erythrocytes incubated in aqueous extracts of <em>Anacardium occidentale, Psidium guajava</em> and <em>Terminalia catappa</em> for 12 h. At regular time intervals of 3 h, portions of the incubation mixtures were withdrawn and spectrophotometric method was used to assay for levels of erythrocyte malondialdehyde (MDA) and methaemoglobin (Met.Hb%). The control analysis showed that within the experimental time, erythrocyte MDA increased from 2.45±0.35 to 3.13±0.59 mmol/ml (<em>p</em> &gt; 0.05; <em>p</em> value = 0.801176). Erythrocyte MDA concentrations in the presence of the three extracts were higher than the control samples at t = 3 h (<em>p</em> &gt; 0<em>.05; p value = 0.963253). Compared with the control samples at the given time (t) intervals, extract of T. catappa exhibited</em> <em>the highest capacity to cause reduction of erythrocyte MDA</em> <em>([T. catappa] = 800 mg%; [MDA] = 2.89±0.33 mmol/ml; t =</em> <em>12 h). Erythrocyte Met.Hb% increased from 2.42±0.55 to 2.51±0.49% (p &gt; 0.05; p value = 0.995171) in the control samples</em> <em>within 12 h.</em> Incubation of sickle erythrocytes with extract of [<em>P. guajava</em>] = 800 mg% for 9 h caused reduction of Met.Hb% from 2.49±0.49% to 2.29±0.45% (p &gt; 0.05; p value = 0.983519). Extracts of <em>A. occidentale, P. guajava and T. catappa</em> exhibited variable capacities to hinder lipid peroxidation, but did not cause corresponding reduction in erythrocyte Met. Hb%, as shown by negative correlation between the two oxidative stress indicators in the presence of <em>T. catappa and higher concentrations of A. occidentale and P. guajava.</em></p> ER -