@article{Hasaniya_Premaratne_Zhang_Razzuk_McNamara_Dashwood_2018, title={Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH}, volume={8}, url={https://www.antiox.org/index.php/fra/article/view/42}, DOI={10.5530/fra.2018.2.11}, abstractNote={<p><strong>Objective:</strong> Hydrogenation of the stable free radical, 2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl (OXANO•), converts it to the intracellular spin trap, 2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine (OXANOH). <strong>Methods</strong>: Due to the flammable nature of the reaction, this procedure must be carried out with extreme care. OXANO• (10 mM) was diluted in 50 ml of Krebs-Henseleit buffer (pH 7.4) and hydrogenated in a nitrogen atmosphere in the presence of Platinum (IV) Oxide Hydrate (100 mg). <strong>Results:</strong> The reaction was completed in approximately 45 min, and was confirmed by the disappearance of the three-electron paramagnetic resonance (EPR) signals (1:1:1). Additionally, the absorption spectra were measured for both OXANOH and OXANO• in the ultraviolet (UV) and visible light regions. <strong>Conclusion:</strong> OXANOH is an extremely valuable oxygen free radical spin trap due to its ability to trap both extra and intracellular free radicals in biological tissues.</p>}, number={2}, journal={Free Radicals and Antioxidants}, author={Hasaniya, Nahidh W and Premaratne, Shyamal and Zhang, Wayne W and Razzuk, Aziz M and McNamara, (Late) J Judson and Dashwood, Roderick H}, year={2018}, month={Apr.}, pages={70–73} }