Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH


Free Radicals and Antioxidants,2018,8,2,70-73.
Published:April 2018
Type:Original Article

Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH

Nahidh W Hasaniya*1, Shyamal Premaratne2,4, Wayne W Zhang3, Aziz M Razzuk4, (Late) J Judson McNamara4, Roderick H Dashwood5

1Department of Cardiovascular Surgery, Kaiser Permanente Medical Group, Fontana, California, USA.

2Hunter Holmes McGuire Veterans Administration Medical Centre, Richmond, Virginia, USA.

3Department of Surgery, University of Washington and VA Puget Sounds Healthcare System, Seattle, Washington, USA.

4Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA.

5Director, Center for Epigenetics and Disease Prevention, Texas A and M College of Medicine, Houston, Texas, USA.


Objective: Hydrogenation of the stable free radical, 2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl (OXANO•), converts it to the intracellular spin trap, 2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine (OXANOH). Methods: Due to the flammable nature of the reaction, this procedure must be carried out with extreme care. OXANO• (10 mM) was diluted in 50 ml of Krebs-Henseleit buffer (pH 7.4) and hydrogenated in a nitrogen atmosphere in the presence of Platinum (IV) Oxide Hydrate (100 mg). Results: The reaction was completed in approximately 45 min, and was confirmed by the disappearance of the three-electron paramagnetic resonance (EPR) signals (1:1:1). Additionally, the absorption spectra were measured for both OXANOH and OXANO• in the ultraviolet (UV) and visible light regions. Conclusion: OXANOH is an extremely valuable oxygen free radical spin trap due to its ability to trap both extra and intracellular free radicals in biological tissues.

About Journal

Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs. About Journal : Free Radicals and Antioxidants [ISSN: 2231-2536] is an online peer reviewed publication dedicated to publishing novel, original findings in the field of free radical biology and both natural and synthetic antioxidants. Free radicals, which are defined as molecules containing one or more unpaired electrons in their outmost atomic or molecular orbital have emerged as double edged swords.The Free Radicals and Antioxidants Journal aims to foster the dissemination of scientific information by publishing manuscripts that provide new insights into the biology of free radicals, including their generation, degradation, modulation, role in cellular function and cell signaling. The submission of uninvited expert reviews and research articles in all areas of Free Radical Biology and Antioxidants are also welcomed. Free Radicals and Antioxidants publishes full research papers presenting original, high quality research, critical review articles providing comprehensive analysis of research development within a defined area and editorial commentaries on key topical issues in Free Radical and Antioxidant Biology.
Distinctions:  The most widely read, cited, and known Free Radicals and Antioxidants and website is well browsed with all the articles published. More than 20,000 readers in nearly every country in the world each month
Editorial Board
Subjects Covered : Natural Products, Free Radical Biology, Antioxidants
ISSN : 2231-2536 ; Frequency : Half Yearly - Rapid at a time publication (2 issues/year)
Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online  publication is 45 days.
Open Access Journal: Free Radicals and Antioxidants is an open access journal, which allows authors to fund their article to be open access from publication.