Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH

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Abstract
Free Radicals and Antioxidants,2018,8,2,70-73.
Published:April 2018
Type:Original Article
Authors:
Author(s) affiliations:

Nahidh W Hasaniya*1, Shyamal Premaratne2,4, Wayne W Zhang3, Aziz M Razzuk4, (Late) J Judson McNamara4, Roderick H Dashwood5

1Department of Cardiovascular Surgery, Kaiser Permanente Medical Group, Fontana, California, USA.

2Hunter Holmes McGuire Veterans Administration Medical Centre, Richmond, Virginia, USA.

3Department of Surgery, University of Washington and VA Puget Sounds Healthcare System, Seattle, Washington, USA.

4Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA.

5Director, Center for Epigenetics and Disease Prevention, Texas A and M College of Medicine, Houston, Texas, USA.

Abstract:

Objective: Hydrogenation of the stable free radical, 2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl (OXANO•), converts it to the intracellular spin trap, 2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine (OXANOH). Methods: Due to the flammable nature of the reaction, this procedure must be carried out with extreme care. OXANO• (10 mM) was diluted in 50 ml of Krebs-Henseleit buffer (pH 7.4) and hydrogenated in a nitrogen atmosphere in the presence of Platinum (IV) Oxide Hydrate (100 mg). Results: The reaction was completed in approximately 45 min, and was confirmed by the disappearance of the three-electron paramagnetic resonance (EPR) signals (1:1:1). Additionally, the absorption spectra were measured for both OXANOH and OXANO• in the ultraviolet (UV) and visible light regions. Conclusion: OXANOH is an extremely valuable oxygen free radical spin trap due to its ability to trap both extra and intracellular free radicals in biological tissues.