An in vitro Study of the Antioxidant and Antiproliferative Properties of Artemisia absinthium- A Potent Medicinal Plant


Free Radicals and Antioxidants,2018,8,1,18-25.
Published:October 2017
Type:Original Article

An in vitro Study of the Antioxidant and Antiproliferative Properties of Artemisia absinthium- A Potent Medicinal Plant

Mohd Yasin Bhat1, Mir Zahoor Gul1, Laxman Ram Lohamror1, Insaf Ahmed Qureshi2, Irfan Ahmad Ghazi1*

1Department of Plant Sciences, 2Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Prof. C. R. Rao Road, Gachibowli, Hyderabad, 500046, Telangana, INDIA.


Background: Artemisia absinthium is a valuable medicinal plant which has vast ethnopharmacological significance. Traditionally, it has been used to cure various ailments like fever, jaundice and other gastrointestinal problems in humans of ancient periods. Objective: The aim of the present study was to evaluate antioxidant and anti-proliferative properties of this medicinal plant. Methods: Total phenolics as well as flavonoid content in different extracts were estimated. Antioxidant activity was evaluated by metal (copper and iron) chelating ability and scavenging of free radicals (DPPH, ABTS, Nitric oxide, Hydroxyl, etc.). 3-(4, 5-Dimethylthiazol- 2-yl)-2, 5-Diphenyltetrazolium Bromide (MTT) assay was done for evaluation of the antiproliferative ability of the extracts. The Fourier Transform Infrared (FTIR) analysis was performed to identify the functional groups present in the metabolites in the different extracts. Results: Among all the extracts, Phenolic and flavonoid content was highest in the hydroalcoholic extract. Different extracts showed variability in total antioxidant activity and free radical scavenging activity. Hexane and methanolic extracts were shown to possess significant antiproliferative activity in a concentration-wise manner. FTIR results revealed the presence of amines, carboxylic acids, aldehydes, ethers, hydroxyl groups and amides which indicate the large variability of metabolites present in the extracts apart from phenolics and flavonoids. Conclusion: The present study validates that A. absinthium is abundant in phytochemicals with natural antioxidants and anticancer activity. It can be a valuable source of anticancer drugs as well as a useful ingredient of pharmaceutical preparations for the treatment of diseases caused by oxidative stress.

About Journal

Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs. About Journal : Free Radicals and Antioxidants [ISSN: 2231-2536] is an online peer reviewed publication dedicated to publishing novel, original findings in the field of free radical biology and both natural and synthetic antioxidants. Free radicals, which are defined as molecules containing one or more unpaired electrons in their outmost atomic or molecular orbital have emerged as double edged swords.The Free Radicals and Antioxidants Journal aims to foster the dissemination of scientific information by publishing manuscripts that provide new insights into the biology of free radicals, including their generation, degradation, modulation, role in cellular function and cell signaling. The submission of uninvited expert reviews and research articles in all areas of Free Radical Biology and Antioxidants are also welcomed. Free Radicals and Antioxidants publishes full research papers presenting original, high quality research, critical review articles providing comprehensive analysis of research development within a defined area and editorial commentaries on key topical issues in Free Radical and Antioxidant Biology.
Distinctions:  The most widely read, cited, and known Free Radicals and Antioxidants and website is well browsed with all the articles published. More than 20,000 readers in nearly every country in the world each month
Editorial Board
Subjects Covered : Natural Products, Free Radical Biology, Antioxidants
ISSN : 2231-2536 ; Frequency : Half Yearly - Rapid at a time publication (2 issues/year)
Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online  publication is 45 days.
Open Access Journal: Free Radicals and Antioxidants is an open access journal, which allows authors to fund their article to be open access from publication.