Free-radical Scavenging properties and Cytotoxic Activity Evaluation of Latex C-serum from Hevea brasiliensis RRIM 600

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Abstract
Free Radicals and Antioxidants,2017,7,1,107-114.
Published:August 2016
Type:Original Article

Free-radical Scavenging properties and Cytotoxic Activity Evaluation of Latex C-serum from Hevea brasiliensis RRIM 600

Leandra Ernst Kerche-Silva1, Dalita Gomes Silva Morais Cavalcante1, Caroline Silva Danna1, Andressa Silva Gomes1, Iriana Moratto Carrara2, Alessandra Lourenço Cecchini2, Eidi Yoshihara3, Aldo Eloizo Job1*

1Departamento de Física, Química e Biologia, Faculdade de Ciências e Tecnologia/UNESP, Presidente Prudente, SP, 19060-080, BRAZIL.

2Departamento de Patologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, 86.057-970, BRAZIL.

3Agência Paulista de Tecnologia dos Agronegócios (APTA), Presidente Prudente, SP, 19015-970, BRAZIL.

Abstract:

Background: There are few studies exploring the antioxidant properties of the latex C-serum of the Hevea brasiliensis latex and their possible use for therapeutic application. Objective: This study investigated the in vitro antioxidant activity of the latex C-serum of H. brasiliensis and assessed the cytotoxicity of this fraction in two human cell lines derived from normal tissue (MRC-5 and CCD 1059sk. Chemical characterization was also assessed. Methods: Latex from H. brasiliensis was centrifuged and the C-serum phase was tested for in vitro free radical scavenging assays, such as hydroxyl and nitric oxide radicals, hydrogen peroxide and total antioxidant capacity by the phosphomolibdate method. Latex C-serum was also investigated for cytotoxic properties by the MTT test. Latex C-serum was chemical characterized by Infrared Spectroscopy Fourier Transform (FTIR). Results: Latex C-serum effectively scavenged hydroxyl and nitric oxide radicals and hydrogen peroxide and presented potent antioxidant activity. The results were compared to ascorbic acid, a standard antioxidant. The cytotoxicity evaluation of latex C-serum demonstrated cell line dependency and for CCD 1059sk cells derived from skin fibroblasts, latex C-serum was not cytotoxic. FTIR showed the presence of proteins in latex C-serum. Conclusion: Latex C-serum from Hevea brasiliensis presented strong antioxidant potential and these results openthe possibility of further studies aiming the use of latex C-serum as treatment for skin diseases related to reactive oxygenspecies or skin preventive pharmacological formulations.

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Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs. About Journal : Free Radicals and Antioxidants [ISSN: 2231-2536] is an online peer reviewed publication dedicated to publishing novel, original findings in the field of free radical biology and both natural and synthetic antioxidants. Free radicals, which are defined as molecules containing one or more unpaired electrons in their outmost atomic or molecular orbital have emerged as double edged swords.The Free Radicals and Antioxidants Journal aims to foster the dissemination of scientific information by publishing manuscripts that provide new insights into the biology of free radicals, including their generation, degradation, modulation, role in cellular function and cell signaling. The submission of uninvited expert reviews and research articles in all areas of Free Radical Biology and Antioxidants are also welcomed. Free Radicals and Antioxidants publishes full research papers presenting original, high quality research, critical review articles providing comprehensive analysis of research development within a defined area and editorial commentaries on key topical issues in Free Radical and Antioxidant Biology.
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