Metabolite Fingerprinting and Antioxidant Potential of Tartary Buckwheat- an Underutilized Pseudocereal crop from Kashmir Region

groupview

Abstract
Free Radicals and Antioxidants,2017,7,1,95-106.
Published:August 2016
Type:Original Article

Metabolite Fingerprinting and Antioxidant Potential of Tartary Buckwheat- an Underutilized Pseudocereal crop from Kashmir Region

Tanveer Bilal Pirzadah1, Bisma Malik1, Inayatullah Tahir2, Mohammad Irfan Qureshi3, Reiaz Ul Rehman1*

1Department of Bioresources, University of Kashmir, Srinagar, Jammu and Kashmir, INDIA-190006.

2Department of Botany, University of Kashmir, Srinagar, Jammu and Kashmir, INDIA-190006.

3Proteomics and Bioinformatics Lab, Department of Biotechnology, Faculty of Natural Sciences, Jamia Millia Islamia, New Delhi, INDIA-110025.

Abstract:

Objective: Buckwheat is an underutilized pseudocereal crop used as a staple food especially in Himalayan regions. The aim of the present study was to evaluate the phytochemical screening, antioxidant potential and metabolite profiling of tartary buckwheat extract. Methods: Tartary buckwheat leaf samples were tested for total phenols, flavonoids and in vitro antioxidant potential in terms of total antioxidant activity, free radical scavenging (superoxide, hydrogen peroxide) and DPPH assay. GC-MS profiling was done to identify and quantify the various metabolites from the methanolic leaf and groat extract. Results: Preliminary phytochemical screening of methanolic extract revealed the presence of alkaloids, flavonoids, phenols, tannins, saponins, phlobatannins, coumarins, glycosides and anthoquinones. Methanolic extract exhibits higher TPC (28.32 ± 5.31 mg gallic acid equivalent g-1 DW) and TFC (25.18 ± 3.5 mg rutin equivalent g-1 DW). DPPH radical (EC50=1.8 μg ml-1) and H2O2 scavenging (EC50=0.103 μg ml-1) potential of tartary buckwheat leaf extract shows promising results. From GC-MS metabolite fingerprinting, over 111 and 24 metabolites were identified among leaf and groat extract respectively. The major compounds present in the extracts were fatty acids, hydrocarbons, steroids, terpenoids, esters, organic acids and aldehydes with excellent pharmaceutical properties. Conclusion: The tartary buckwheat extract were found to contain numerous metabolites with potent antioxidant and other pharmacological actions. Thus, tartary buckwheat could be a promising alternative in functional food sector to improve social well-being and neutraceutical to diminish malnutrition especially for the impoverished community.

About Journal

Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs. About Journal : Free Radicals and Antioxidants [ISSN: 2231-2536] is an online peer reviewed publication dedicated to publishing novel, original findings in the field of free radical biology and both natural and synthetic antioxidants. Free radicals, which are defined as molecules containing one or more unpaired electrons in their outmost atomic or molecular orbital have emerged as double edged swords.The Free Radicals and Antioxidants Journal aims to foster the dissemination of scientific information by publishing manuscripts that provide new insights into the biology of free radicals, including their generation, degradation, modulation, role in cellular function and cell signaling. The submission of uninvited expert reviews and research articles in all areas of Free Radical Biology and Antioxidants are also welcomed. Free Radicals and Antioxidants publishes full research papers presenting original, high quality research, critical review articles providing comprehensive analysis of research development within a defined area and editorial commentaries on key topical issues in Free Radical and Antioxidant Biology.
Distinctions:  The most widely read, cited, and known Free Radicals and Antioxidants and website is well browsed with all the articles published. More than 20,000 readers in nearly every country in the world each month
Editorial Board
Subjects Covered : Natural Products, Free Radical Biology, Antioxidants
ISSN : 2231-2536 ; Frequency : Half Yearly - Rapid at a time publication (2 issues/year)
Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online  publication is 45 days.
Open Access Journal: Free Radicals and Antioxidants is an open access journal, which allows authors to fund their article to be open access from publication.