Comprehensive Assessment Of Antioxidant Activities of Apigenin Isomers: Vitexin and Isovitexin


Free Radicals and Antioxidants,2016,6,2,155-166.
Type:Original Article

Comprehensive Assessment Of Antioxidant Activities of Apigenin Isomers: Vitexin and Isovitexin

Swati Khole1, Niranjan A Panat1, Priyanka Suryawanshi1, Suchandra Chatterjee2, TPA Devasagayam3, Saroj Ghaskadbi1

1Department of Zoology, SPPU, Pune-411007, Maharashatra, INDIA.

2Food and Technology Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400085, Maharashtra, INDIA.

3Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400085, Maharashatra, INDIA.


Introduction: Fenugreek is known to possess anti-diabetic as well as antioxidant properties known to increase with germination. Previous study from our laboratory has reported vitexin and isovitexin as major antioxidant compounds from germinated fenugreek seeds. The present study aimed to comprehensively assesses and compare their antioxidant activity. Methods: The antioxidant ability of these compounds against various radicals was checked using standard in vitro radical scavenging assays and pulse radiolysis. Protection conferred to mitochondria against oxidative damage was assessed by measuring the levels of lipid peroxides and protein sulphydryls. Protection from hydrogen peroxide induced cytotoxicity in HepG2 cells was checked by MTT assay and by measuring intracellular ROS. Their ability to modulate intracellular antioxidant status was checked both at activity and RNA level. Result: Vitexin and isovitexin exhibited differential antioxidant activity against various radicals. Vitexin was a better nitric oxide scavenger while isovitexin scavenged superoxide radicals more efficiently. Pulse radiolysis studies revealed good antioxidant activity of both compounds against short-lived radicals and affected the formation and decay of the 2-dG transient species to the same extent. In oxidatively damaged mitochondria, lipid peroxidation was inhibited significantly by isovitexin whereas vitexin prevented decrease in protein sulphydryl content more effectively. These compounds protected HepG2 cells to the same extent against hydrogen peroxide induced oxidative insult by reducing intracellular ROS and modulating the levels of antioxidant enzymes. Conclusion: The present study clearly demonstrates differential antioxidant potential of both vitexin and isovitexin and their role in mitigating oxidative stress induced damage and maintaining cellular redox homeostasis.

About Journal

Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs. About Journal : Free Radicals and Antioxidants [ISSN: 2231-2536] is an online peer reviewed publication dedicated to publishing novel, original findings in the field of free radical biology and both natural and synthetic antioxidants. Free radicals, which are defined as molecules containing one or more unpaired electrons in their outmost atomic or molecular orbital have emerged as double edged swords.The Free Radicals and Antioxidants Journal aims to foster the dissemination of scientific information by publishing manuscripts that provide new insights into the biology of free radicals, including their generation, degradation, modulation, role in cellular function and cell signaling. The submission of uninvited expert reviews and research articles in all areas of Free Radical Biology and Antioxidants are also welcomed. Free Radicals and Antioxidants publishes full research papers presenting original, high quality research, critical review articles providing comprehensive analysis of research development within a defined area and editorial commentaries on key topical issues in Free Radical and Antioxidant Biology.
Distinctions:  The most widely read, cited, and known Free Radicals and Antioxidants and website is well browsed with all the articles published. More than 20,000 readers in nearly every country in the world each month
Editorial Board
Subjects Covered : Natural Products, Free Radical Biology, Antioxidants
ISSN : 2231-2536 ; Frequency : Half Yearly - Rapid at a time publication (2 issues/year)
Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online  publication is 45 days.
Open Access Journal: Free Radicals and Antioxidants is an open access journal, which allows authors to fund their article to be open access from publication.