Total Phenolic, Flavonoid and Alkaloid Contents, Oxidative DNA Damage Protective and Antioxidant Properties of Methanol and Aqueous Extracts of Dissotis rotundifolia Whole Plant

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Abstract
Free Radicals and Antioxidants,2018,8,2,82-88.
Published:April 2018
Type:Original Article

Total Phenolic, Flavonoid and Alkaloid Contents, Oxidative DNA Damage Protective and Antioxidant Properties of Methanol and Aqueous Extracts of Dissotis rotundifolia Whole Plant

Williams Kweku Darkwah1, 2*, Yanhui Ao1, Micheal Buenor Adinortey2, Alexander Weremfo2, Francis Kwame Abrokwah2, and Emmanuel Afriyie3,2

1Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Environmental Engineering Department, College of Environment, Hohai University, CHINA.

2Department of Biochemistry, School of Biological Sciences, University of Cape Coast, Cape Coast, GHANA.

3Department of Bioscience, University of Milan, Milan, ITALY.

Abstract:

Background: Antioxidants present in natural sources helps to scavenge free radicals and thus, provide health benefits. The main objective for this research is to determine phenolic, flavonoid, and alkaloid contents, nitric oxide scavenging activity, hydroxyl radical scavenging activity, and DPPH-scavenging activity, ferric reducing power of the extracts and DNA damage protecting activity to evaluate the antioxidant potential of various extracts. Materials and Methods: In vitro antioxidant potential of the D. rotundifolia extract was evaluated using 1, 1‑diphenyl‑2‑picrylhydrazyl (DPPH), nitric oxide (NO), and hydroxyl (OH) radical scavenging assays. Ferric reducing power ability of the extract was also examined using tannic acid, and ascorbic acid as standard. Concentrations of plant extracts ranging from 0.02 to 0.10 mg/ml were prepared and mixed with appropriate volumes of reagents. Results: Methanol extract of D. rotundifolia exhibited higher content of phytochemical compounds (alkaloid = 12.4 mg QE/g; flavonoids = 19.3 mg QE/g; and phenols = 18 mg QE/g) at concentration 0.1 mg/ml compare to the aqueous extract (alkaloid = 9.1 mg QE/g; flavonoids = 10.5 mg QE/g; and phenols = 16.5 mg QE/g). An over-all trend found in the present study highlights the fact that the methanol extract have better antioxidant capacities (DPPH, NO, OH and FRAP) than the aqueous extract. D. rotundifolia extracts exhibited considerable protection to the damage of native supercoiled circular at concentrations, 0.1 mg/ml and 10 mg/ml. Conclusion: The study showed that the extracts can competently protect the body against oxidative stress, therefore can be used as a source of potent natural antioxidant compounds.

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Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs. About Journal : Free Radicals and Antioxidants [ISSN: 2231-2536] is an online peer reviewed publication dedicated to publishing novel, original findings in the field of free radical biology and both natural and synthetic antioxidants. Free radicals, which are defined as molecules containing one or more unpaired electrons in their outmost atomic or molecular orbital have emerged as double edged swords.The Free Radicals and Antioxidants Journal aims to foster the dissemination of scientific information by publishing manuscripts that provide new insights into the biology of free radicals, including their generation, degradation, modulation, role in cellular function and cell signaling. The submission of uninvited expert reviews and research articles in all areas of Free Radical Biology and Antioxidants are also welcomed. Free Radicals and Antioxidants publishes full research papers presenting original, high quality research, critical review articles providing comprehensive analysis of research development within a defined area and editorial commentaries on key topical issues in Free Radical and Antioxidant Biology.
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